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simian virus 40 large t antigen sv40t  (Addgene inc)


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    Structured Review

    Addgene inc simian virus 40 large t antigen sv40t
    Simian Virus 40 Large T Antigen Sv40t, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/simian+virus+40+large+t+antigen+sv40t/pLenti+CMV%2FTO+SV40+small+%2B+Large+T+(w612-1)+(Plasmid+%2322298)/pmc12162870-316-13-22
    Average 93 stars, based on 57 article reviews
    simian virus 40 large t antigen sv40t - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Collaborative orchestration of BH3-only proteins governs Bak/Bax-dependent hepatocyte apoptosis under antiapoptotic protein-deficiency in mice.
    Article Snippet: Isolated hepatocytes were cultured in William’s eagle medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FCS), 2 mM L-glutamine (Thermo Fisher Scientific), 100 nM insulin (Sigma‒Aldrich) and 100 nM dexamethasone (Sigma‒Aldrich). .. To immortalize mouse primary hepatocytes, they were transfected with a lentiviral vector expressing simian virus 40 large T antigen (SV40T) purchased from Addgene (Watertown, MA, USA, plasmid #22298). .. BNL CL.2 cells were purchased from the American Cell Death & Differentiation Type Culture Collection (ATCC, Manassas, VA, USA).

    Article Title: Collaborative orchestration of BH3-only proteins governs Bak/Bax-dependent hepatocyte apoptosis under antiapoptotic protein-deficiency in mice
    Article Snippet: Isolated hepatocytes were cultured in William’s Eagle medium (Thermo Fisher Scienti c) supplemented with 10% fetal bovine serum (FCS), 2 mM Lglutamine (Thermo Fisher Scienti c), 100 nM insulin (Sigma‒Aldrich) and 100 nM dexamethasone (Sigma‒Aldrich). .. To immortalize mouse primary hepatocytes, they were transfected with a lentiviral vector expressing simian virus 40 large T antigen (SV40T) purchased from Addgene (Watertown, MA, USA, plasmid #22298). .. BNL CL.2 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Article Title: Collaborative orchestration of BH3-only proteins governs Bak/Bax-dependent hepatocyte apoptosis under antiapoptotic protein-deficiency in mice
    Article Snippet: Isolated hepatocytes were cultured in William’s eagle medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FCS), 2 mM L-glutamine (Thermo Fisher Scientific), 100 nM insulin (Sigma‒Aldrich) and 100 nM dexamethasone (Sigma‒Aldrich). .. To immortalize mouse primary hepatocytes, they were transfected with a lentiviral vector expressing simian virus 40 large T antigen (SV40T) purchased from Addgene (Watertown, MA, USA, plasmid #22298). .. BNL CL.2 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Plasmid Preparation:

    Article Title: Collaborative orchestration of BH3-only proteins governs Bak/Bax-dependent hepatocyte apoptosis under antiapoptotic protein-deficiency in mice.
    Article Snippet: Isolated hepatocytes were cultured in William’s eagle medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FCS), 2 mM L-glutamine (Thermo Fisher Scientific), 100 nM insulin (Sigma‒Aldrich) and 100 nM dexamethasone (Sigma‒Aldrich). .. To immortalize mouse primary hepatocytes, they were transfected with a lentiviral vector expressing simian virus 40 large T antigen (SV40T) purchased from Addgene (Watertown, MA, USA, plasmid #22298). .. BNL CL.2 cells were purchased from the American Cell Death & Differentiation Type Culture Collection (ATCC, Manassas, VA, USA).

    Article Title: Collaborative orchestration of BH3-only proteins governs Bak/Bax-dependent hepatocyte apoptosis under antiapoptotic protein-deficiency in mice
    Article Snippet: Isolated hepatocytes were cultured in William’s Eagle medium (Thermo Fisher Scienti c) supplemented with 10% fetal bovine serum (FCS), 2 mM Lglutamine (Thermo Fisher Scienti c), 100 nM insulin (Sigma‒Aldrich) and 100 nM dexamethasone (Sigma‒Aldrich). .. To immortalize mouse primary hepatocytes, they were transfected with a lentiviral vector expressing simian virus 40 large T antigen (SV40T) purchased from Addgene (Watertown, MA, USA, plasmid #22298). .. BNL CL.2 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Article Title: Collaborative orchestration of BH3-only proteins governs Bak/Bax-dependent hepatocyte apoptosis under antiapoptotic protein-deficiency in mice
    Article Snippet: Isolated hepatocytes were cultured in William’s eagle medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FCS), 2 mM L-glutamine (Thermo Fisher Scientific), 100 nM insulin (Sigma‒Aldrich) and 100 nM dexamethasone (Sigma‒Aldrich). .. To immortalize mouse primary hepatocytes, they were transfected with a lentiviral vector expressing simian virus 40 large T antigen (SV40T) purchased from Addgene (Watertown, MA, USA, plasmid #22298). .. BNL CL.2 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Expressing:

    Article Title: Collaborative orchestration of BH3-only proteins governs Bak/Bax-dependent hepatocyte apoptosis under antiapoptotic protein-deficiency in mice.
    Article Snippet: Isolated hepatocytes were cultured in William’s eagle medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FCS), 2 mM L-glutamine (Thermo Fisher Scientific), 100 nM insulin (Sigma‒Aldrich) and 100 nM dexamethasone (Sigma‒Aldrich). .. To immortalize mouse primary hepatocytes, they were transfected with a lentiviral vector expressing simian virus 40 large T antigen (SV40T) purchased from Addgene (Watertown, MA, USA, plasmid #22298). .. BNL CL.2 cells were purchased from the American Cell Death & Differentiation Type Culture Collection (ATCC, Manassas, VA, USA).

    Article Title: Collaborative orchestration of BH3-only proteins governs Bak/Bax-dependent hepatocyte apoptosis under antiapoptotic protein-deficiency in mice
    Article Snippet: Isolated hepatocytes were cultured in William’s Eagle medium (Thermo Fisher Scienti c) supplemented with 10% fetal bovine serum (FCS), 2 mM Lglutamine (Thermo Fisher Scienti c), 100 nM insulin (Sigma‒Aldrich) and 100 nM dexamethasone (Sigma‒Aldrich). .. To immortalize mouse primary hepatocytes, they were transfected with a lentiviral vector expressing simian virus 40 large T antigen (SV40T) purchased from Addgene (Watertown, MA, USA, plasmid #22298). .. BNL CL.2 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Article Title: Collaborative orchestration of BH3-only proteins governs Bak/Bax-dependent hepatocyte apoptosis under antiapoptotic protein-deficiency in mice
    Article Snippet: Isolated hepatocytes were cultured in William’s eagle medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FCS), 2 mM L-glutamine (Thermo Fisher Scientific), 100 nM insulin (Sigma‒Aldrich) and 100 nM dexamethasone (Sigma‒Aldrich). .. To immortalize mouse primary hepatocytes, they were transfected with a lentiviral vector expressing simian virus 40 large T antigen (SV40T) purchased from Addgene (Watertown, MA, USA, plasmid #22298). .. BNL CL.2 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Virus:

    Article Title: Collaborative orchestration of BH3-only proteins governs Bak/Bax-dependent hepatocyte apoptosis under antiapoptotic protein-deficiency in mice.
    Article Snippet: Isolated hepatocytes were cultured in William’s eagle medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FCS), 2 mM L-glutamine (Thermo Fisher Scientific), 100 nM insulin (Sigma‒Aldrich) and 100 nM dexamethasone (Sigma‒Aldrich). .. To immortalize mouse primary hepatocytes, they were transfected with a lentiviral vector expressing simian virus 40 large T antigen (SV40T) purchased from Addgene (Watertown, MA, USA, plasmid #22298). .. BNL CL.2 cells were purchased from the American Cell Death & Differentiation Type Culture Collection (ATCC, Manassas, VA, USA).

    Article Title: Collaborative orchestration of BH3-only proteins governs Bak/Bax-dependent hepatocyte apoptosis under antiapoptotic protein-deficiency in mice
    Article Snippet: Isolated hepatocytes were cultured in William’s Eagle medium (Thermo Fisher Scienti c) supplemented with 10% fetal bovine serum (FCS), 2 mM Lglutamine (Thermo Fisher Scienti c), 100 nM insulin (Sigma‒Aldrich) and 100 nM dexamethasone (Sigma‒Aldrich). .. To immortalize mouse primary hepatocytes, they were transfected with a lentiviral vector expressing simian virus 40 large T antigen (SV40T) purchased from Addgene (Watertown, MA, USA, plasmid #22298). .. BNL CL.2 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Article Title: Collaborative orchestration of BH3-only proteins governs Bak/Bax-dependent hepatocyte apoptosis under antiapoptotic protein-deficiency in mice
    Article Snippet: Isolated hepatocytes were cultured in William’s eagle medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FCS), 2 mM L-glutamine (Thermo Fisher Scientific), 100 nM insulin (Sigma‒Aldrich) and 100 nM dexamethasone (Sigma‒Aldrich). .. To immortalize mouse primary hepatocytes, they were transfected with a lentiviral vector expressing simian virus 40 large T antigen (SV40T) purchased from Addgene (Watertown, MA, USA, plasmid #22298). .. BNL CL.2 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).



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